Part:BBa_K3610016:Design

SmallBit NanoLuc - codon optimized for S. cerevisiae

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.

Source

The sequence was taken of Part:BBa_K3168003 from the iGEM registry and then codon optimized with SnapGene.

References

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.

Trotta, Edoardo (2013): Selection on codon bias in yeast: a transcriptional hypothesis. In: Nucleic Acids Res 41 (20), S. 9382–9395. DOI: 10.1093/nar/gkt740.